Sequencing Primer Design

Course Overview

Primer design is a critical step in sequencing and molecular biology experiments, determining the accuracy and efficiency of downstream analysis. This workshop provides both theoretical foundations and hands-on training in designing primers for different sequencing applications, including singleplex, multiplex, and next-generation sequencing (NGS). Led by Yustinus Maladan, S.Si., M.Si. (Pusat Riset Biologi Molekuler Eijkman), participants will explore principles of primer design, tools and resources available, and practical strategies for in silico PCR and primer pool design. This workshop is ideal for students, researchers, and professionals who want to strengthen their molecular biology and sequencing skills. Primer design is a critical step in sequencing and molecular biology experiments, determining the accuracy and efficiency of downstream analysis. This workshop provides both theoretical foundations and hands-on training in designing primers for different sequencing applications, including singleplex, multiplex, and next-generation sequencing (NGS). Led by Yustinus Maladan, S.Si., M.Si. (Pusat Riset Biologi Molekuler Eijkman), participants will explore principles of primer design, tools and resources available, and practical strategies for in silico PCR and primer pool design. This workshop is ideal for students, researchers, and professionals who want to strengthen their molecular biology and sequencing skills.

Sub-topics

• 1.
  Theoretical Background: Introduction to Primer Design for Sequencing, Principles of Primer Design, Singleplex vs Multiplex Primer Design, Primer Design Tools & Resources

• 2.
  Hands-On: Primer Design for Singleplex PCR, Primer Pool Design for Multiplex PCR, In Silico PCR, Primer Pool Applications for NGS

Course Components

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