Introduction to DNA Sequencing and Analysis

Course Overview

This online course offers a beginner-friendly introduction to modern DNA sequencing technologies and their analysis. It starts with an overview of key sequencing methods, with a special focus on Whole Exome Sequencing (WES) — a powerful approach for studying genetic variations in coding regions of the genome. Participants will learn the basic workflow of DNA-Seq analysis, from raw data processing to variant calling and interpretation. The course also covers essential tools and file formats used in DNA-Seq analysis, such as FASTQ, BAM, VCF, and tools like BWA, GATK, and IGV. A guided hands-on session will walk learners through a simplified WES analysis pipeline, providing practical experience in handling sequencing data. This course is ideal for students, researchers, and professionals looking to build a foundational understanding of DNA sequencing and analysis. No prior experience with sequencing analysis is required, though basic knowledge of molecular biology is recommended. This online course offers a beginner-friendly introduction to modern DNA sequencing technologies and their analysis. It starts with an overview of key sequencing methods, with a special focus on Whole Exome Sequencing (WES) — a powerful approach for studying genetic variations in coding regions of the genome. Participants will learn the basic workflow of DNA-Seq analysis, from raw data processing to variant calling and interpretation. The course also covers essential tools and file formats used in DNA-Seq analysis, such as FASTQ, BAM, VCF, and tools like BWA, GATK, and IGV. A guided hands-on session will walk learners through a simplified WES analysis pipeline, providing practical experience in handling sequencing data. This course is ideal for students, researchers, and professionals looking to build a foundational understanding of DNA sequencing and analysis. No prior experience with sequencing analysis is required, though basic knowledge of molecular biology is recommended.

Sub-topics

• 1.
  Introduction to DNA Sequencing Technologies

• 2.
  What is Whole Exome Sequencing (WES)?

• 3.
  DNA-Seq Analysis Workflow Overview

• 4.
  Common Tools & File Formats

• 5.
  Hands-on Session: WES Analysis Pipeline

Course Components

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